In the selection of binders from a library and their affinity maturation, a trade-off between affinity of the binder and its stability frequently can be observed. Starting from a binder library based on a very stable framework allows for high sequence diversity in the randomised positions of the putative binding site, and therefore stringent selection for high affinity, as very significant destabilisation would be needed to bring the stability of the binder down to a level where this stability significantly affects the fraction of folded molecules and therefore the outcome of selection.
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I encounter a series problem when running this demo, and past my questions as follows:
(A) About the README file problem, At the second step, I think the command is not right, Is the "@finder_option" should be replaced with "@maker_options" ?
2) Make the potential homodimers
There are two ways to do this. If you are only doing it for one structure is is easy just to use this command line
I am playing around with the interface optimization script in the demo "design_raf_rac_interface" (rosetta3.5). For the system I am working with, I would like to add a few residues to the list of residues to be redesigned, an not redesign all residues of the interface, but only those around a mutated residue in the binding partner.
I plan to design a small protein fragment using flexible linkers (to connect the breaks) out of a larger protein.
Using which rosetta application i should use to put the residues of the flexible linkers to get the complete 3D structure of the shorter protein.
Please Help me!
Thanks in advance
Hi, I used fixbb in Rosetta 3.4 to thread a sequence on a template structure. There is a disulfide bond in the template structure, say, between two residues Nr. 20 and 50. My resfile looks like this: NATAA # keep native aa but packable! ... 20 A PIKAA G ... 50 A PIKAA G ... However, in the threaded model, all mutations specified in the resfile were realized except the two cystine residues. How can I force fixbb to mutate the two cystine residues?