Design

Hello, i am a masters student trying to play around with rosetta. And i have a project in mind. I have two protein structures: nanocages. each with 60 monomers but the chains are a little bit different with different aa profiles. Now what I want to do is to make a structure with the backbone conformation profile of one of the nanocages but with the amino acid profile of the other one. How do i go about doing this? I'm sorry if I wasn't clear english is not my native language. 

Thanks for your help in advance. 

Hello all,

I am keen on creating a custom Antibody design protocol for a problem.

I have a 4 chain, 96 residues long PDB file. all four chains are identical, so I set up my rosettascript with the following indices to select each chain to mutate them.

<Index name="chain1" resnums="1-24"/>

<Index name="chain2" resnums="24-48"/>

<Index name="chain3" resnums="48-72"/>

<Index name="chain4" resnums="72-96"/>

However I get the follwoing error message when running this script: 

I have been trying to insert residues into a PDB and then relaxing with Rosetta using rosettascripts. Currenlty I have managed a way to replace specified residues with another direct subsitution, but can;t figure out how to directly add more residues. I'm attempting another method by manually adding "dummy" residues into the pdb and then having rosetta replace those with the desired reisdue.  Would it be something accomplished using a resfile instead of a Rosettascripts function?

<ROSETTASCRIPTS>

<SCOREFXNS>