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What should I do to lower down the fa_sol scores?

Category: 
Scoring

Greetings,

I ran a design protocol and I find my results score has high fa_sol scores like 250 around. What should I do to lower down the fa_sol scores? 

I used ResidueSelector to separate my alpha-helixs into three parts: buried, semi-buried and solvent exposure. And use resfiles to assign the amino acids, buried------hydrophobic, semi-buried------less hydrophobic, solvent exposure-------hydrophilic. 

And I am wondering if the fa_sol score of a model is too high, will it be insoluble in water after experimental expression?

Post Situation: 

Score.sc file showing total score 0 for all protein structures created

Category: 
Scoring

Hello, I am trying to use Rosetta to design the interface of my protein and would like to rank the predicted structures based on their scores however, after running the job, all the files show 0 for the total score. Could anyone take a look at my xml script and flags file (in the pdf file) to see if I'm missing something or how else to fix this issue? Thanks in advance!

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Error with sequence_tolerance.R processing the checkpoint files, *.ga.entities and *.ga.generations.

Category: 
Scoring

Hello,

the output files, *.ga.entities and *.ga.generations, were produced by sequence_tolerance application. 

When I tried to use sequence_tolerance.R processing the output files, I got the following error:

> source("/usr/local/rosetta_src_2021.16.61629_bundle/main/tools/analysis/apps/sequence_tolerance.R")

> process_seqtol("./2I0L_A_C_V2006_0001.ga.entities")

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ERROR: Unable to open weights/patch file

Category: 
Scoring

Hello,

I am getting the following error:

ERROR: Unable to open weights/patch file. None of (./)ref2015 or (./)ref2015.wts or /cluster/rosetta/bin/mpi/../database/scoring/weights/ref2015 or /cluster/rosetta/bin/mpi/../database/scoring/weights/ref2015.wts exist

Post Situation: 

Per residue total score

Category: 
Scoring

Hi Rosetta Team!

I read from the scoring tutorial this line

A rule of thumb: -1 to -3 REU per residue is typical while scoring a refined structure with ref2015 score function.

May I know if it is possible to directly call this per residue total score during scoring?

Thank you.

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