ddg_monomer: what is the theoretical solution condition for the scoring?
As I understand, the Gibbs free energy not only depends on the structure but also depends on the surrounding solution conditions. That is why under certain conditions (e.g. pH, temperature, ionic strength), the protein tends to unfold or remain native structure. So I wonder if there is a theoretical assumption to set the scoring background? For example, is it under pH 7, 25 degree C, or vacuum state? Thank you.