Symmetry

Hello,

I am trying to design a C3 symmetrical protein with a ligand bound at the C3 interface. What is the best way around this in RosettaScripts? I was thinking to first symmetrize the pose with SetupForSymmetry and then add the ligand with AddChain mover. But it seams, SetupForSymmetry is not compatible with extra_res. As soon as the script arrives at SetupForSymmetry, it throws the following error:

Dear All,

I would like to simultaneously dock and design two (or more) identical subunits into a a symmetric homooligomer.

Input:

- backbone structure of a monomer (no sidechains!);
- initial arrangement of the subunits;
- constraints that precisely define interactions between the subunits.

Simulation:

Hello,

Multiple input structures are provided as a list (in:file:l) or in a single silent file (in:file:silent).

Is there an easy way to provide also symmetry definition files for the individual structures? (symmetry:symmetry_definition)

Thanks you for suggestions!

Best wishes,

Staszek

Dear all

I was trying to mix ligand docking with protein design of a symmetric dimer by using the SetupNCS mover. I still don't know why the mover is not being used...the resultant pdbs were modified at the sequence level but both chains are different.

thanks in advance

felipet

The script used is:

        <MOVERS>

                <SetupNCS name=ncs bb=1 chi=1 symmetric_sequence=1>