Unsolved

Hi,

I would like to know whether the enzyme design protocol of Rosetta Suite can be used for increasing the catalytic activity of the enzyme. Here, I don't have to design the active site from scratch, but I need to design the active site in such a way so that the catalytic activity of the enzyme is increased. Also, the enzyme I am dealing with does not have data for transition state modeling for the substrate that I am dealing with.

Please provide you comments and suggestions

Thanks
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BH

Hi everyone,

I'm trying to run Rosetta kinematic loop on my computer but I got an error message after few seconds:

ERROR: Cannot open PDB file "input_pdb"
ERROR:: Exit from: src/core/import_pose/import_pose.cc line: 184

The comand that I have used and the screenshot of the error page is shown below:

Hi all,

I would like to make a change to the source code of a particular application - it's a very minor, but necessary, change that should make the application more broadly applicable.

However, having made the change in the .cc file - how would I go about recompiling the code so that it links to the rest of the Rosetta package, or is the simplest way just to recompile the whole package?

Thanks in advance.

wsg

When running the Loop Modeling script on my protein of interest, the identity of the amino acids in the loop changes when it generates the full atom model from the centroid model. In other words, in the centroid model the amino acids are equivalent to the protein sequence from the original PDB, but they are "mutated" in the FA model (often to lots of G's). Is there a way to prevent this from happening?