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Unable to download Rosetta

Category: 
Phenix / MR Rosetta

Hello my name is Yair Tenorio from

Genetics and Molecular Biology Department at CINVESTAV México

I already have an account, I am registered and logged in, but when I try to

download Rosetta with my User Name and Password, is not letting me download the software

I hope you could help me, my domain is @cinvestav.mx

the web page tells me to verify my email, which I already did.

 

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NMR NOE based structure prediction

Category: 
PyRosetta

Based on the NMR NOE data, I want to use PyRosetta for the structure prediction of my peptides having unnatural amino acids. I went through the manual but couldn't find much information for structure prediction based on NMR data. It would be of great help if you cite protocols, scripts or literature used to determine the structure using NMR data in PyRosetta.

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Academic email verification

Category: 
ROSIE

Good afternoon,

I am a student at IPSI (Institució Pedagògica Sant Isidor) in Barcelona and I am currently looking forward to use Rosettadock. However, my email is not recognised as an academic one. My email is victor.calatayud.07@ipsi.cat, the domain being ipsi.cat. If you could verify it I would be very grateful. 

Thanks

Víctor Calatayud,

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m6A modification on FARFAR2

Category: 
ROSIE

Hi everyone,

I been trying to work with FARFAR2 and I found documentation Fasta File (rosettacommons.org), which talks about possibilities of modified nucleotides on our RNA model; however, I could not find anything about m6A modification. I feel like there must be something for m6A given that it is most prevalent modification on RNA. 

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Altered pLDDT scores after running FastRelax on AlphaFold Multimer output pdb files

Category: 
PyRosetta

Hello,

I hope this message finds you well. I noticed after running FastRelax on output pdb files from AlphaFold Multimer that the pLDDT scores encoded in the B-factor fields have been altered. Is there a way to avoid this and maintain the original pLDDT scores? I have attached the code I am running below. 

Thank you,

Franz

Post Situation: 

Replacing a single residue in a structure

Category: 
Design

I have a structure i have been working with for a while, and I wish to replace the last residue in the sequence with a cysteine. I have tried to write a xml script that would perform the spot mutation, but I could not figure it out. Is there a specific rosetta function or way to perform spot mutations? I have also considered just manually replacing the residue with cysteine, and relaxing the structure through rosetta, but i'm not sure if the cysteine would stay in the sequence.

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