I'm fairly new to using Rosetta suite, so sorry if silly questions. I'm running ddg_monomer app for a protein of about 150 aa.
A) the pdb outputs I get are all the same, there is no backbone or sidechain movement neither for wt nor for mut structures (50 for each as recommended). I intended to carry out the protocol of row 16 in Kellogg, 2011. I attached the options file, if you'd be so kind to take a look at it. What am I missing here?
B) I'm working in a 2GB RAM PC. After a while, I got the process Killed (just that in shell prompt), and I guess it was due to memory overload, I checked memory consumption. The process starts over 45% memory usage and ramps to 65% in a half hour when the process stops without completion. Can anyone tell me what I'm doing wrong, or I just need a better PC?