I used the Rosetta Design server (http://rosettadesign.med.unc.edu) to search for mutations that stzabilize a protein-protein interface.
I have a two questions about this:
1) Is there any controll on the process, e.g., by means of a .res residue file, as is for the structure stabilization procedure? I seems like the settings in the .res file to not influence the PPIface stabilization procedure at all.
2) Is it possible to extract/reproduce the structure of the mutated protein complex, e.g., by using the protein stabilization protocol on this server with specific settings in the .res file ... and how could such settings look like in order to reproduce the structure for an interface stabilizing single-point mutation.
Thanks a lot,