# Which function can be use for mutation?

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Which function can be use for mutation?
#1

Dear friends:

I want mutate the particular residues of protein or peptide with PFB forma. Which function or program can be used in pyrosetta?

Thanks a lot.

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Wed, 2017-10-18 09:10
sprinkle166

Probably the easiest way to mutate a residue is to use the pyrosetta.toolbox.mutate_residue() function. That should work if you just want a simple change of residue identity (to one of the standard 20 amino acids) and aren't two picky about it.

For a little more control, you'll want to read up on resfiles, the packer, and repacking/design.

If you're doing something with residues that aren't part of the the normal 20 amino acids, then the packer approach may work, but you might need to do other things, depending on what you're interested in accomplishing.

I will admit, though, that I'm not quite sure what you're looking for, or what "PFB forma" means. Perhaps if you expand upon what you're attempting to accomplish, I might be able to give more specific suggestions.

Thu, 2017-10-19 12:03
rmoretti

rmoretti, thank your very much! I'll try it.

Thu, 2017-10-19 12:57
sprinkle166