I want to manually set protonation of residues.
I find there are params in Rosetta database folder
What are differences between ASP_P1.params and ASP_P2.params, and GLU_P1.params and GLU_P2.params ?
In additon, HIS has two deprotonation states, HID and HIE, why there are only one file HIS_P.params ?
The above problem pluzzes me for a long time,