I performed p_mut_scan to see if certain mutants make the protein more stable. Is it absolutely necessary to run Relax (1) before running anything  like pmut_scan or ddg_monomer. I have already done about 6 months of work with these without Relax. Surprisingly, pmut_scan did correctly predict 2 mutants.
-> Do I have to redo everything since I did not relax the structure?
->Another question: What is the meant by "options_annealer" section in the documentation: " By default, fixbb uses the standard annealer used"