I want to design the interface of a two-chain complex( actually design only one of the partner) using raf / rac design script which I have attached it here.
Is it better and more accurate to change this script in order to get and use resfile ?
I prefer to design interface automatically without resfile (just script defines interface residues) because I have no idea about which residues in interface should be designed or not. or what types of mutations are allowable
Would it be a serious problem if I want to NOT use resfile? for example, I worry about disturbing protein structure completely due to too many mutations