I'm trying to to use rosetta design (http://rosettadesign.med.unc.edu) to increase binding affinity between 2 proteins in 1 pdb file.
I want 2 residue ranges to be mutated in chain B but all other residues to remain the same. Therefore I have used this resfile:
71 - 88 B ALLAAxc EX1
50 - 56 B ALLAAxc EX1
However, the server mutates residues in both chains. How do I force it to only mutate chain B?