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multi state protein design

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multi state protein design
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Hello,

I have an enzyme and I want to optimize its sequence to increase its binding to one protein and decrease its binding to another protein at the same time. I recently attended Rosetta workshop and learn about multi state protein design which can be used to increase affinity of an antibody to its ensemble of antigen, but I am not sure whethe it is applicable to my case. I would appreciate any suggestions.

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Fri, 2020-09-04 08:14
rohi

Rosetta has at least two multistate design tools.

mpi_msd (https://www.rosettacommons.org/docs/latest/application_documentation/design/mpi-msd) (https://www.rosettacommons.org/demos/latest/protocol_capture/multistate_apl/README) will definitely work for this purpose.  You can set up explicitly negative states for the decreased binding desired.

RECON probably will as well.

Fri, 2020-09-04 11:25
smlewis