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the question about ddg of disulfide bond mutants

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the question about ddg of disulfide bond mutants
#1

I want to evaluate the ddg between WT and  disulfide bond mutants 

1. I try to use ddg_monomer and fond it does not suport it

2. Then I build disulfide bond mutants with moddler, and then  try to use ddg_monomer to evaluate  the ddg ( mutant  CYS  recovery to the amino acid of WT), and it does not work , too.

3. Just as 2.  I try to use another APP, cartesian_ddg , and  disulfide bond mutants as the star model,and  ddgs seem is similar(just try three mutant)

 

How can I evaluate the ddg between WT and  disulfide bond mutants ? Thanks

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Sun, 2021-08-29 06:40
asbelx

Could someone please answer my question?

Wed, 2021-09-08 23:19
asbelx