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Unexpected length of light chain FR1

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Unexpected length of light chain FR1

Hello Rosetta forum,

I recently started using rosetta for building antibody structures and running "antibody.linuxgccrelease -fasta L2251x0566aa.fasta | tee grafting.log" command on my sequences. Seems like the default setting doesn't prefer light-FR1 length >22 ? Any help would be appreciated.

File: src/protocols/antibody/grafting/
ERROR: Unxpected length of light-fr1 [length=23], length expected to be: [19, 20, 21, 22]!

Look forward to some responses.

Thank you,


Post Situation: 
Fri, 2022-12-09 01:03