I would like to compare the affinity between 2 difference structures. InterfaceAnalyzer can calculate binding energy with more suitable by including dSASA term. In the description dG_separated/dSASAx100: Separated binding energy per unit interface area * 100 to make units fit in score file. Scaling by dSASA controls for large interfaces having more energy. The factor of 100 is to allow standard 2.45 notation isntead of something like 2.45E-2. When look in the detail, dSASA is calculated from the difference between binding complex and separated complex. So, the larger interface, the greater dSASA, right?. Therefore, the dG should be higher(get closer to zero) when divided by large interface or high dsasa score. It does not make sense to me . Please anyone give me the right explaination.