Dear Professor Bryan Der,
Thank you very much for your great server. I've come across two essential questions using your techniques and I really need your help so I can continue working on my PhD thesis.
I've used both supercharge methods:
1- In terms of the Rosetta method , I assigned my "Target Net Charge Protein" to be negative. I was wondering if I need to disable (uncheck or untick) the rosetta positive supercharge options including ARG-LYS and enable (check or tick) the Rosetta negative supercharge options including ASP-GLU?
2- In order to use the AVNAPSA-Negative method, do I need to disable (untick) both the options of Rosetta positive supercharge( including ARG-LYS) and Rosetta negative supercharge (including ASP-GLU) ? What I mean is that do I have to untick the boxes for ASP-GLU-ARG-LYS?
Would you please assist me in this matter? Thank you so much in advance for your advise and assistance.It's highly appreciated.