David Baker in this video at minute number 15:34 talks about having a standard, easy, and routine way to design novel (synthetic) proteins that do not exist in nature. So routine that even new students can successfully and easily preform it.
Questions: Is Rosetta Design the protocol to acheive this? Is there a full document from start to finish that details this protocol? (the one in the demos details a recetpor dimer protein). From the talk I understood that an Abinitio step comes after the Design step, is there a refinement step somewhere? Is there another step that results in very low score/RMSD funnel shaped plots? Are there certain preffered starting structures? Is there any details about the probability of a successful crystal structure after a good Abinitio result?
I am looking for the details please, but any information will be valuable.
Appologies for the mass of questions, but no one arround me here is doing this work.