Dear ROSETTA users,
for a current iGEM competition research project, we are considering using ROSETTA for protein structure predictions. However, as we have very limited experience with the software, we do not know how to start the computation.
For our project, we aim at evolving the Methanocaldococcus jannaschii tRNA-synthetase for the non-canonical amino acid nitrophenylalanine, and would like to simulate the process before doing it in practice. We were wondering whether there are any further information available online with regards to the simulation processes (e.g. protocols, helpful guides) for our purposes, or if anyone has done something similar and could provide us with protocols or scripts.
Thank you very much for your help!
ROSETTA: how to run simulations with non-canonical amino acids?. Available from: https://www.researchgate.net/post/ROSETTA_how_to_run_simulations_with_non-canonical_amino_acids [accessed Jun 8, 2017].