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Error in score funtion "hack_elec 0.42"

Category: 
Docking

Hello everyone!

I am getting this following error when I am trying to run a rosettaDNA application program in which i want to change the specificity of a protein so that it binds to a target DNA. In most published articles, I have seen "hack_elec 0.42 " or similar values. However, it's not working in my case. Can you help me with this?

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Academic email recognition

Category: 
ROSIE

Hello, I am a postdoc at the Max Planck Istitute for Chemical Ecology and would to use ROSIE for acedemic purposes. My email adress scarr@ice.mpg.de is not recognized.

I posted this same request a few weeks ago and got no responce. Any help would be greatly apreaciated. Cheers

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How to use rewrite_rosetta_script.py to update XML script file?

Category: 
Design

Hello!

I am trying to run the default XML script to change the specificity of a protein so that it binds to a DNA sequence of interest. For this I am referring to the book Redesigning the specificity of protein-DNA interactions with Rosetta

Thyme S, Baker D. Redesigning the specificity of protein-DNA interactions with Rosetta. Methods Mol Biol. 2014;1123:265-82. doi: 10.1007/978-1-62703-968-0_17. PMID: 24510272.

However I am getting the following error message.

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