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Fragment Generation

make_fragments.pl error

Category: 
Fragment Generation

Hello,

I am trying to run make_fragments.pl but I get this error:

FILENAME: lig_0.fasta
Sequence: DDYLWGLEAGEGISDLFD
Running sparks for phi, psi, and solvent accessibility predictions
running psiblast for sequence: lig_0.fasta
[blastpgp] WARNING:  [000.000]  posPurgeMatches: Due to purging near identical sequences, only the query is used to construct the position-specific score matrix

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How to concatenate the 3-mer and 9-mer files for RosettaCM?

Category: 
Fragment Generation

I am now working on a protein with multiple chains, and the total amino acids exceeds 1000. So I need to submit each chain one by one, get the individual 3-mer and 9-mer fragments, then concatenate them.

http://old.robetta.org/fragmentsubmit.jsp

The attachment is the 3-mer file. After concatenating them, do I only need to adjust these three places?

I will then use the 3-mer and 9-mer for the RosettaCM.

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Is there other online server for generate fragment?

Category: 
Fragment Generation

Hi,Is the old robetta server under maintenance now?

I want generate 3-mer and 9-mer fragment but i can not find this service in new robetta ,so i use old robetta instead.But now it seems does not work.

Does any server other than the old robetta have this function?

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Is native.pdb necess for fragment_picker?

Category: 
Fragment Generation

Hi,i want to use fragment_picker to get 3-mer and 9-mer fragment,with only .fasta and .psipred.Cause i do not have pdb file ,i remove the -in::file::s .pdb

When i run fragment_picker,it tell me "can not read a referrence file"

I want to know how to solve this,thank you 

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Make error during installation of updated ncbi-blast-2.9.0+-src plus error with nr-database

Category: 
Fragment Generation

Dear fellows,

my recent situation with setting up the fragment generation for structure prediction ab initio consists in follow:

- It seems as if I solved my problems with version compatibility of gcc-compiler and the environment variable FRAGMENT_PICKER.

- But a problem with installation of updated make_fragments.pl and install_dependencies.pl is still remained unsolved. I carried out next actions with the ncbi-blast-2.9.0+-src package:

Post Situation: 

kcenters_clustering_of_fragments

Category: 
Fragment Generation

Hello Rosetta community,

I'm having trouble finding the fragment generation application "kcenters_clustering_of_fragments." The application is mentioned as publically available in the methods section of D.A. Silva et al (2019). De novo design of potent and selective mimics of IL-2 and IL-15. Nature. Link: https://www.nature.com/articles/s41586-018-0830-7?WT.feed_name=subjects_computational-biology-and-bioinformatics

Post Situation: 

make_fragments.pl

Category: 
Fragment Generation

Hi everyone,

           I wanted to let you know about a bug that I was able to fix. if you use the make_fragments.pl in the /rosetta/tools/fragments/ folder it calls another script install_dependencies.pl it calls an online script update_blastdb.pl which in the install_dependencies.pl is at this url:

 

https://www.ncbi.nlm.nih.gov/blast/docs/update_blastdb.pl

 

it returns a 404 error. 

 

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