You are here

Unsolved

The problem hasn't been solved

error with fragment_picker.linuxgccrelease

I got the error message when I run fragment_picker:
rosetta_source/bin/fragment_picker.linuxgccrelease: error while loading shared libraries: libprotocols.so: cannot open shared object file: No such file or directory

I think this is because some part of the software didn't compile right, but I don't know where. Could anyone help me with this?
Thank you very much!

Post Situation: 

Possible documentation error

Hi,

I just wanted to report what I think is an error in the documentation for versions 3.3 & 3.4.

On the "Using Constraints Files in Rosetta" page, in the function definition for a SPLINE function, the documentation currently says:
"SPLINE: histogram_file_path experimental_value weight bin_size ... If using RosettaEPR knowledge-based potential, replace with EPR_DISTANCE"

According to my reading of the source code (and my experience using it!) there is a field missing in that description. It should read something like:

Post Situation: 

Energy and RG in the new rosetta3.4 ( like RG_Energy.cc and RG_Energy_Fast.cc )

Hello

I have a huge database of PDB files and I need to calculate their RG along with their energy(any relevant scoring).
Earlier version of Rosetta had RG_Energy.cc and RG_Energy_Fast.cc type of files which did this kind of job.

Is there something similar for Rosetta3.4 ?
Please advise

Post Situation: 

symmetric Docking- TRIMER in Rosetta 3.4 ?

I need to do certain symmetric docking to generate its homodimer and homotrimer and I am using Rosetta 3.4 Symmetric docking to achieve it.

I used the following command to generate homodimers :

./bin/SymDock.linuxgccrelease -docking:dock_ppk -symmetry:symmetry_definition ./sym_def_dimer.dat -symmetry:initialize_rigid_
body_dofs -database ../rosetta_database/ -out:nstruct 2 -out:file:fullatom -s S_00000001.pdb

Post Situation: 

how to optimize the weights?

Hi,
I found that there are optimize_weights directory in the protocol directory in the rosetta 3.3 and 3.4. What's the function of this protocol and how to use it? I think this is for weights optimization. If I understand correct In this case, if I have 1 more energy term(suppose this term is totally new), how do I use ”optimize_weights“ to optimize the weights? Thank you very much!

Post Situation: 

Single Strand DNA (ssDNA) 3D structure prediction

I have an ssDNA and want to predict its 3D structure. Can I use Rosetta?
The secondary structure of my sequence is attached. For RNA we can use Rosetta to predict 3D structure but unfortunately I could not find any tools that predict tertiary structure of ssDNA? Please guide me, can i simply change Ts to Us, and predict a 3D RNA structure using Rosetta etc and finally in 3D structure replace Us with Ts?

Thank you very much.

Post Situation: 

Pages

Subscribe to RSS - Unsolved