I am not sure if this is the correct forum to ask this question, but I thought many people here would have knowlege to answer me.
So Rosetta designs proteins using normal amino acids such as Methionine (MET), but most proteins are then taken to bacterial system for expression (rather than mammalan/insect expression systems), bacterial systems which use N-Formylmethionine (fMET) as its initiation codon. Some bacterial species would also use Selenomethionine (MSE) instead of Methionine throughout a protein.
Maybe this is a very basic question, but won't these different methionines impact the final structure of the protein? Should I put this issue under considiration when designing proteins? expecially if they were imbedded in the core? Can someone explain to me the general picture, or point me to a resource that explains this?
I hope my question is not too NEWB to be answered.