Hi
I am writing here after so much struggle with incorporation of NCAA (Bpy-Ala) using different rosetta scripts like:
Hello,
I am running a xml script with rosetta_scripts (Rosetta 3.14, v2022.11) in my HPC cluster and I am getting the following error:
Hi Rocco, Steven, et al,
Posting this here since it appears that the Rosetta bugs reporting tool is currently down.
Hi all,
I want to generate cyclic peptides in a binding site employing the anchor design approach similar to https://www.nature.com/articles/s41467-021-23609-8 and https://www.pnas.org/doi/suppl/10.1073/pnas.2012800118. I need to do the closure by amidation between two side chains (K/E) or side chain/C-terminal.
I was trying to use Rosettascript to perform a grafting task. I found that an intermediate pose of my task has the following discontinuity of residue number.
I found that the grafted residues by CCDEndsGraftMover do not have proper indexing. They have no chain ID, and all residue numbers are 0. If the XML option copy_pdbinfo was set to 1, the grafted residues just shared the same chain ID and residue number as the original segment. I am wondering if there are any movers that do the following tasks.
I want to design a repeat protein that self-assembles into a designed structure. As a result, I want each repeat to acquire the same sequence after the FastDesign mover. Is it possible that during FastDesign mover, all repeats are mutated the same way? Is there any Taskoperation (or mover) I can call to set up such a constraint?
Dear researchers,
Those who really want to use the get_fasta_from_pdb.py script to generate the FASTA files from PDB, generally face some sorts of errors.
The most common error is: TabError: inconsistent use of tabs and spaces in indentation
Hi there!
Hello,
I am designing a macrocycle with simple_cycpep_predict that contains a D-Leu in the N-terminal and an acetylated ASP in the C-terminal. My sequence_file (-cyclic_peptide:sequence_file) is DLE ........ ASP
1) When I run the app it doesn't recognize the D-Leu name:
