Design

Hi, everyone, sorry for disturbing! I ran into a error when I ran rifgen program to generate RIF residues for Patchdock.

commands line: $RIFDOCK/build/apps/rosetta/rifgen @input/rifgen.flag > rifgen.log 2>&1

results:

Hi all,

I would like to redesign the dimeric interface using the heterodimer structure of protein A and protein B. I searched several protocols and saw how to increase affinity or stability by fixing one of them and changing the other. However, I want to increase the binding affinity between the two proteins by changing both sides at the dimeric interface. Anyone know how to do that? Has this method been tried before? If there are papers or protocols that I can refer to, please let me know.

Any help will be very appreciated.

Best,

Hi everyone, has anyone used the program protein mimic designer? I imitated the article "De novo design of potent and selective mimics of IL-2 and IL-15" to design IL-2. In the first step, I got a lot of pdb files and stored them in the folder 01_results_scaffoldsMimicsIL2/debug. I I don't know if this result is correct, because there is an error at the end of the program running: segmentation fault (core dump).
If anyone has done it, what is the result of your first step?

The results of my first step

Hello,

I am using Recon MSD. I have run it functionally for a few months but now it is not working. When I try to run my bash script I get an error at the same point every time. It is during the "AddMembrane" step of my Rosetta Scripts protocol. I have tried countless tweaks to my inputs and my scripts but the same segmentation fault happens at the same time every run.