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Cartesian_ddG mutfile error

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Cartesian_ddG mutfile error

hello gus,

When i use catesian-ddg for protein stability, i want saturation site by all amino acied. but I Set  "1 A ALLAA" OR "C 1 ALLAA"(first site is  cys), all error.

and i type "total 9 


C 1 A 

C 1  W 

C 1  F 

C 1  I
.....  " Enumerative method is OK. So, how I set mutfile more easy and clearly ? help!

Post Situation: 
Mon, 2024-02-05 01:25