I want to modeling my antibody by webserver of rosetta antiboy, but I faced with the error:
ERROR: Current DB does not cover the length of FRL of your query. ERROR: FRL length of your query: 59 ERROR: DB: 58 or 60
Would you help me to solve the error?
The antibody code chops the FASTA sequence up into subsections, for the various constant regions and the CDR loops. One of those regions is abbreviated "FRL" - probably framing region light, the not-CDRs in the light chain.
The database (DB) only has 58 and 60 residue options for that region. You've submitted a FASTA that it thinks has 59 residues in that region.
I guess the easy solution is to try deleting or adding a residue someplace innocous (the termini?) to see if you can get it to 58 or 60 so it will run?