I have an rRNA pdb file (up to few hundred residues) with missing segments of residues (from 3 to 10 residues in one missing segment; about 10 segments ) and I need to add(predict) the missing parts of structure and get a complete pdb.
1)Is it possible to do it by PyRosetta?
2)- I installed pyrosetta and I'm trying to follow the workshops to get more familiar with rosetta more-less successful, but when I tried to load some RNApdb I got an error also how could I use method pose_from_sequence with A, G, C, U?- Is there any tutorial or help how to do it?
3) (trying to answer 1) I hoped I could load my pdb then insert missing parts using pose_from_sequence and
insert_pose_into_pose then set MoveMap to change only inserted residues and then use some movers something like simple de novo folding algorithm in Workshop4
I appreciate any help you can provide.