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error while building rosetta++

Hello

I am new to rosetta. When i am trying to build rosetta by typing make gcc,i am getting a long message a part of which looks like this:

src/utility/io/zipstream.ipp:657: instantiated from ‘void zlib_stream::basic_zip_ostream::add_footer() [with Elem = char, Tr = std::char_traits, ElemA = std::allocator, ByteT = unsigned char, ByteAT = std::allocator]’

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Username and Password for download link?

Hello,

I applied for the express academic license for pyrosetta and received the license email very rapidly. However, as compared to the Rosetta license where I also received an email with the download link and username and password, I did not receive such for Pyrosetta. Any ideas?

Thanks so much,

Judy

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excecutable files don't work -- Rosetta3.2 on WindowsXP

Hi
I tried installing rosetta 3.2 on windows XP using cygwin1.7, gcc 3.4.4. following a procedure posted on 10/20/2009.

It seems I was able to compile rosetta3.2. There are "executable files" in rosetta_source/bin folder and rosetta_source/build/src/release/cygwin/1.7/32/x86/gcc folder. They look like AbinitioRelax.cygwingccrelease, backrub.cygwingccrelease and so on.

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Fragment files for membrane_abinitio application

Hi

I am trying to make fragment files for membrane_abinitio application. I have secondary structure prediction results (*.rdb files) from SAM secondary structure server. I am trying to run the command:
make_fragments.pl -nojufo -nopsipred -noprof -samfile HRG3_all_X.t2k.str2.rdb HRG3.fasta

The program is generating all the files (.cst, .a2m) but somehow its not generating fragment (frag3, frag9) files at all.

Could anyone please help in this matter ?

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How to estimate the effect of a mutation on the binding energy?

Given a protein complex, I would like to estimate the effect of a point mutation on the binding energy (ddG). I'd appreciate if anyone could point me to the Rosetta app(s) that I should look into. I'm fairly new to Rosetta.

Thanks!

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how to relax structure constraining CA in Rosetta3.2?

Hi

I am wondering how to relax structure constraining CA in Rosetta3.2?

I found that each protocol contains two executable command such as:

relax.default.linuxgccrelease relax.linuxgccrelease

is there any differences between these two?

THX

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Fragment files for membrane_abinitio application

Hi

I am trying to make fragment files for membrane_abinitio application. I have secondary structure prediction results (*.rdb files) from SAM secondary structure server. I am trying to run the command:
make_fragments.pl -nojufo -nopsipred -noprof -samfile HRG3_all_X.t2k.str2.rdb HRG3.fasta

The program is generating all the files (.cst, .a2m) but somehow its not generating fragment (frag3, frag9) files at all.

Could anyone please help in this matter ?

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is it possible to ab initio only a part of a protein?

I've built a homology model but a part of it (around 12aa)is not reliable because there is no homology template in this region. The whole protein is around 350aa. And I am wondering is it possible built a ab init model based on my initial model to complete these unreliable region?

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How to design a stand-alone loop from a binding interface?

Hi, Dear colleagues!

I am new to Rosetta and am now finding whether there is a solution to my problem using powerful Resetta.

I want to cut a loop which is responsible for the binding of A to B from protein A. I need to constrain this loop so it will maintain its bound conformation though it is now in the apo form.

The problems are:
1) I need to find a way to automatically constrain it either by using disulfide bond and cyclization;
2) The loop is disulfide-bonded to the rest of the protein and this disulfide bond may be responsible for maining a turn in its bound conformation;

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